Molecular Biology

DNA Extraction

  • Whole Blood, serum, plasma,
  • Saliva
  • Urine
  • Buccals
  • Formalin-fixed paraffin-embedded (FFPE) slide

RNA Isolation

  • Blood
  • FFPE

PCR, Purification, Enzyme Digestion, Electrophoresis

  • Primer design
  • Primer synthesis
  • Validation of PCR primers
  • PCR (regular, high-fidelity, GC-Rich)
  • PCR purification
  • Agarose Gel Electrophoresis
  • Enzyme digestion

DNA Mutation detection by Sequencing or Real Time PCR

  • Sequencing method (see PCR section; sequencing will be charged by CHOP NAP core)
  • qPCR run

Gene Expression (mRNA) analysis (TaqMan real time RT-PCR)

  • qRT-PCR run (price varies with number of genes and number of samples, please inquire)